Electric Cigarette (ENDS) and conventional contains carbon monoxide (CO) which is a major cause of cardiovascular disease. CO is a dangerous substance since as free radicals it have high affinity for hemoglobin. Thus affinity could lead to tissue hypoxia resulting in cell death (necrosis) started by the cell nucleus pyknosis. In order to further study about the effect, this experiment is conducted to identify the histopathological appearence of the left ventricle rat against exposure to electric cigarette (ENDS) and conventional. This study was conducted with a post test only control group design method. Samples contained 18 Rattus novergicus rat which was divided in 3 groups: control group (K) as a negative control, P1 group were exposed by conventional cigarretes, and P2 group were exposed by ENDS. The treatment had been maintained for 30 days, followed by termination to perform the heart organ dissection and HE staining in order to made preparat. Preparat was observed by 400x lens of microscope to identify the pyknosis of the nuclei in 5 field of view. Data were analyzed with oneway ANOVA test with p<0,05 and CI=95%. This study proved that there was a significant differences of pycnotic nuclei amount among the group (p-value=0,008). There was significant difference between group K with P1 (p=0,004), group K with group P2 (p=0,012). There was no significant difference between group P1 and P2 (p=0,567).It can be conluded that exposure to either electric cigarette (ENDS) or conventional cigarette gives significant effect to cell nucleus pycnosis. Meanwhile, the effect comes from exposure of electric cigarette smoke (ENDS) has no significant difference with conventional cigarette.

Author Name: Yanuarita Tursinawati Tursinawati, Noor Yazid Yazid, Findi Wira Purnawati

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Keywords: histopathological appearance of left ventricle, electrical cigarette, ENDS, conventional cigarette

ISSN: 2541-2272

EISSN: 2548-9526

EOI/DOI: http://dx.doi.org/10.30651/qm.

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