OBJECTIVE: Cytomegalovirus is considered to be one of the most serious pathogens affecting immunosuppressed individuals particularly HIV/AIDS and glycoprotein B (gB) is essential for viral infectivity as they play a role in attachment and penetration of the host cells during viral transmission and fusion into infected cells. This study was conducted to determine the molecular diversity of gB glycoproteins of CMV strains distribution among HIV positive patients in Federal Medical Centre Bida. METHODS: Blood samples were collected for IgM ELISA and CD4+ cell counts. Viral DNA extraction was carried out on all IgM positive samples and genotyping of glycoprotein B gene (UL 55 region) was detected by multiplex nested PCR. RESULTS: Out of the three hundred and eighty five (385) HIV positive subjects, a prevalence of 19.8% CMV IgM was found among patients with HIV in Bida. PCR revealed that gB1 was more prevalent (83.7%) followed by gB2 (16.3%) among subjects. The absence of mixed infection confirmed that there was no case of reinfection as all the primary infections were cases of reactivation of CMV infection among HIV patients. CONCLUSION: This study found significant relationship in marital status, location and educational status with gB genotype distribution in Bida. Although, there was paucity of reports on CMV gB genotype distribution with socio demographic factors and behavioral data, this study provided baseline information on CMV molecular diversity of CMV genotypes gB1 and gB2 among HIV positive patients in Nigeria, more studies on CMV glycoprotein B genotype from Nigeria may help to determine the optimal strains for CMV vaccine development in Nigerian population.

Author Name: Omosigho OP, Okojie RO, Tatfeng YM

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Keywords: Cytomegalovirus, Glycoprotein B, HIV/AIDS, HAART, Bida

ISSN: 1116-1043

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