Tissue culture techniques, one of the biotechnological applications for the protection of plant biodiversity, are often used especially in the protection of endemic plants. Iris sari Schott ex Baker, an endemic species of the family Iridaceae, has natural spreading areas in our country. The aim of our study is to investigate the vegetative multiplication and effective rooting possibilities of Iris sari Schott ex Baker, one of the geophytes of Turkey, with in vitro techniques. For this purpose, immature embryos belonging to the Iris sari Schott ex Baker plant have been used as initial explant. Rooting studies were done with shoots obtained as a result of in vitro proliferation studies. As a result of the studies, the best rate of reproduction was observed in MS medium containing 0.5 mg l-1 NAA + 1.0 mg l-1 BAP with 10.3 shoots per explant. In rooting of proliferated plantlets, the medium in which the highest rooting is achieved was found in MS medium containing 1.0 mg l-1 IBA+2.0 mg l-1 JA with 16.5 roots per plant. When the bulb diameters of plantlets were examined, it was observed that the best bulb development was achieved from MS medium containing 1.0 mg l-1IBA+0.2 mg l-1NAA+2.0 mg l-1JA and 1.0 mg l-1IBA with a bulb diameter increase average of 4.19 mm.

Author Name: Selay DO?AN

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Keywords: Iris sari Schott ex Baker, in vitro propagation, rooting, JA, bulb formation.

ISSN: 1300-0225

EISSN: 2667-6087

EOI/DOI: 10.18615/anadolu.727197

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