Sunflower (Helianthus annuus L.), is oil crop plant belonging to Compositae (Asteraceae) family and is the fourth in the world in terms of the largest consuming edible oil and approximately 60% of the sunflower cultivation areas in the world are in the Black Sea Region countries. Sunflower is growing almost all parts of Turkey due to higher adaptation capability. The amount of vegetable oil produced in Turkey is not enough for our domestic consumption and this existing deficit is increasing due to increasing population year by year. This deficit could be reduced by improving of oil quality and the use of higher oleic acid could decrease the consumption of frying oil. The selection utilizing with classical breeding methods to develop varieties containing high oleic acid is both hard and less precision because it is affected more by biotic and abiotic stress conditions. However, it is possible to obtain faster and more consistent results in plant breeding containing high oleic acid by using biotechnological methods and selections supported by molecular marker selection (MAS). In this study, the screening of 40 F3 individuals and also 55 higher, mid and linoleic acid content type cultivars have been screened by molecular markers and analyzed for fatty aside contents. Six INDEL markers based on the variations of FAD2 gene region that is related to high oleic acid trait and 3 SSR markers that were reported as high oleic linked markers in the literatures were used in this study. All of the samples were analyzed by gas chromatography for detection of their fatty acid contents and compared to molecular marker results. As a result of this study, 3 markers which can be used for selection of the high oleic trait were detected.

Author Name: Ça?lar ÇOLAK; Semra HASANÇEB?; Yalç?n KAYA

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Keywords: Sunflower, Helianthus annuus L., oleic acid, molecular marker, MAS, SSR, INDEL, gas chromatography.

ISSN: 1300-0225

EISSN: 2667-6087

EOI/DOI: 10.18615/anadolu.727207

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