Jamblang is a tropical plant that is widely grown in Indonesia. According to preclinical studies, jamblang plants have benefits as an antioxidants, anti-inflammatory, anti-cancer, and antibacterial. The content of active compounds in jamblang leaves can act as an antibacterial agent against Staphylococcus auereus and Streptococcus mutans in others research. The purpose of this study is to identify the antibacterial activity and toxicity of the jamblang leaves extract against Staphylococcus epidermidis bacteria. The method used in this experiment is Minimum Inhibitory Concentration (MIC) method using disc diffusion and Brine Shrimp Lethality Test (BSLT) for toxicity testing. The research used ethanol extract of jamblang leaves [Syzygium cumini (L.) Skeels] with various concentrations (20%, 40%, 60% and 80%), the test bacteria used was pure cultures of Staphylococcus epidermidis, and 30 µg of chloramphenicol as the positive control, 10% DMSO solution as the negative control. A phytochemical test was conducted to determine the content of active compounds in jamblang leaves extract. The results showed that the ethanol extract of jamblang leaves contained flavonoids, tannins, saponins, and steroids with a total flavonoid content was 1.38%. The toxicity test using BSLT method was resulted LC50 value is 485.50 ppm which indicated that the plant extract is toxic, and if value of LC50 was below 1000 ppm its indicate as toxic properties. The result of MIC value was obtained with a level of concentration at 20% and a 9 mm diameter inhibition zone therefore was categorized as moderate antibacterial based on Clinical and Laboratory Standard Institute (CSLI).

Author Name: Nurmeily Rachmawati, Gina Maulidiyah, & Aminah

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Keywords: Ekstrak daun jamblang, Konsentrasi hambat minimum, Staphylococcus epidermidis, toksisitas

ISSN: 0854-4425

EISSN: 2338-834X

EOI/DOI: 10.47349/JBI/17012021/39

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