Abiotic stress is one of the major rate limiting factor for rice production worldwide especially in Asia and Africa. While drought tolerance is become of increasing importance in rice (Oryza sativa L.), selection under actual field conditions is tiresome due to low heritability and time required. Selection in tissue culture is thought to be one way to improve selection efficiency, but this requires standardized protocols. Rice cultivars BRRI 28 and BRRI 29 showed expressive callus induction, but the ability for callus induction and regeneration decreased under mannitol stress in both cultivars. Calli were induced on semisolid Murahige and Skoog (MS) medium supplemented with 2 m g/l 2,4-dichlorophenoxyacetic acid (2 ,4 -D). Embryogenic calli showed shoot regeneration on MS medium supplemented with 2 mg/l benzyl aminopurine (BAP ) + 0. 5 mg/l kinetin + 0.5 mg/l naphthalene acetic acid (NAA). Increased levels of mannitol (0 gm/l, 10 gm/l, 20 gm/l, 30 gm/l, 40 gm/l) were used to create drought stress. There was reduction in callus induction ability and plant regeneration efficiency with increasing levels of mannitol stress.These results indicated that mannitol can be used as drought stress creating agent under in vitro conditions and rice variety BRRI 29 was relatively tolerant to drought stress as compared to BRRI 28. This study will serve as a base line for in vitro screening of drought tolerant transgenic rice.

Author Name: Zahidul Haque, Anindita chakraborty, Shamsul H. Prodhan

URL: View PDF

Keywords: IJSER,2229-5518,22295518,Zahidul Haque, Anindita chakraborty, Shamsul H. Prodhan

ISSN: 22295518

EISSN:

EOI/DOI:

Add Citation Views: 4322

---