The main objective of the present investigation was to analyse phytochemical constituents and preparation of silver nanoparticles from the flower extracts of Mesua ferrea, and their antibacterial activity. The aqueous and methanol extracts of flowers were prepared using water and methanol as solvents. The phytochemical analysis of the extracts was carried out by following standard protocols. The flower extract showed the presence of alkaloids, terpenoids, resins and tannins. The dried flower powder was mixed with the aqueous solution of 1mM silver nitrate solution and synthesized silver nano particles by incubating it at 95ºC for one hour. The colour change in the solution from light brown to dark brown colour was due to surface plasmon vibration. Maximum absorption peak was observed at 358 nm and the bio-molecules involved in the reduction of silver ions was confirmed by Fourier Transform Infrared spectroscopy (FTIR) and the size of nanoparticles was observed by SEM study. The synthesized silver nanoparticles were hexagonal in shape with the diameter range of 80-120nm. The diameter size confirms the presence of silver nanoparticles. Anti-microbial activity was carried out with the aqueous, methanol and AgNO3 floral extracts against 7 bacterial species which were Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae,Pseudomonas aeruginosa, Salmonella sp. and Streptococcus sp. The aqueous extract was found to be more susceptible to all the organisms than the methanol and AgNO3 extract.The methanolic extract showed that at 150?l it had maximum zone of inhibition for all the tested bacterial strains, while 50 ?l showed lower zone of inhibition. Therefore, the silver nano particles from the flower extract can be used for treatment of diseases caused by bacterial pathogens.
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Author Name: N. Lakshmi, E. Jenitta Emima Packiyam, P. Rama Bhat and K. Jayade
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Keywords: Mesua ferrea, silver nanoparticles, FTIR, SEM, antibacterial activity
ISSN:
EISSN: 2278-3229
EOI/DOI: NIL
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